Title:
An Electrically Active Microneedle Electroporation Array for Intracellular Delivery of Biomolecules

dc.contributor.advisor Allen, Mark G.
dc.contributor.author Choi, Seong-O en_US
dc.contributor.committeeMember Mark R. Prausnitz
dc.contributor.committeeMember Oliver Brand
dc.contributor.committeeMember Pamela Bhatti
dc.contributor.committeeMember Shyh-Chiang Shen
dc.contributor.department Electrical and Computer Engineering en_US
dc.date.accessioned 2008-02-07T18:12:02Z
dc.date.available 2008-02-07T18:12:02Z
dc.date.issued 2007-11-14 en_US
dc.description.abstract The objective of this research is the development of an electrically active microneedle array that can deliver biomolecules such as DNA and drugs to epidermal cells by means of electroporation. Properly metallized microneedles could serve as microelectrodes essential for electroporation. Furthermore, the close needle-to-needle spacing of microneedle electrodes provides the advantage of utilizing reduced voltage, which is essential for safety as well as portable applications, while maintaining the large electric fields required for electroporation. Therefore, microneedle arrays can potentially be used as part of a minimally invasive, highly-localized electroporation system for cells in the epidermis layer of the skin. This research consists of three parts: development of the 3-D microfabrication technology to create the microneedle array, fabrication and characterization of the microneedle array, and the electroporation studies performed with the microneedle array. A 3-D fabrication process was developed to produce a microneedle array using an inclined UV exposure technique combined with micromolding technology, potentially enabling low cost mass-manufacture. The developed technology is also capable of fabricating 3-D microstructures of various heights using a single mask. The fabricated microneedle array was then tested to demonstrate its feasibility for through-skin electrical and mechanical functionality using a skin insertion test. It was found that the microneedles were able to penetrate skin without breakage. To study the electrical properties of the array, a finite element simulation was performed to examine the electric field distribution. From these simulation results, a predictive model was constructed to estimate the effective volume for electroporation. Finally, studies to determine hemoglobin release from bovine red blood cells (RBC) and the delivery of molecules such as calcein and bovine serum albumin (BSA) into human prostate cancer cells were used to verify the electrical functionality of this device. This work established that this device can be used to lyse RBC and to deliver molecules, e.g. calcein, into cells, thus supporting our contention that this metallized microneedle array can be used to perform electroporation at reduced voltage. Further studies to show efficacy in skin should now be performed. en_US
dc.description.degree Ph.D. en_US
dc.identifier.uri http://hdl.handle.net/1853/19710
dc.publisher Georgia Institute of Technology en_US
dc.subject Inclined UV lithography en_US
dc.subject Electroporation en_US
dc.subject Microneedles en_US
dc.subject Metal transfer en_US
dc.subject Micromolding en_US
dc.subject.lcsh Drug delivery devices
dc.subject.lcsh Transdermal medication
dc.subject.lcsh Skin absorption
dc.subject.lcsh Electroporation
dc.title An Electrically Active Microneedle Electroporation Array for Intracellular Delivery of Biomolecules en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.corporatename School of Electrical and Computer Engineering
local.contributor.corporatename College of Engineering
relation.isOrgUnitOfPublication 5b7adef2-447c-4270-b9fc-846bd76f80f2
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
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