Lateral gene transfer of pandemic Vibrio parahaemolyticus genomic island genes

Silberger, Daniel James

Title:

Lateral gene transfer of pandemic Vibrio parahaemolyticus genomic island genes

dc.contributor.author	Silberger, Daniel James
dc.contributor.department	Biology
dc.date.accessioned	2008-12-18T19:19:41Z
dc.date.available	2008-12-18T19:19:41Z
dc.date.issued	2008-12-15
dc.description.abstract	Vibrio parahaemolyticus is an emerging enteric pathogen. The O3:K6 serotype has become pandemic, and the genome has been sequenced for the RIMD2210633 strain of this serotype (20). The thermostabile direct hemolysin (tdh), thermostabile related hemolysin (trh), and two distinct Type III Secretion Systems (T3SS) have been implicated in virulence. Deletion studies have determined that the T3SS alone are sufficient to induce eukaryotic cell apoptosis. The T3SS loci are found within two separate genomic islands. Because genomic islands are known to be disseminated by Lateral Gene Transfer (LGT), we examined the distribution of the pandemic genomic island genes among clinical and environmental V. parahaemolyticus isolates and also among closely-related environmental Vibrio spp. using primers designed to amplify hemolysins and T3SS effectors previously characterized for V. parahaemolyticus strain RIMD2210633 (23). We also examined the distribution of a hypothetical bacteriocin, encoded by vpa1263, within another genomic island that is similar to the bacteriocins produced by Escherichia coli. Preliminary screens for the bacteriocin gene suggest that many clinical strains and some environmental strains contain vpa1263. PCR screens for the T3SS effector genes have shown that 22% of closely-related environmental Vibrio spp. contain at least one T3SS1 or one T3SS2 effector gene. Sequencing of housekeeping genes is ongoing to confirm these findings.
dc.description.advisor	Brian Hammer - Committee Member/Second Reader ; Patricia Sobecky - Faculty Mentor
dc.identifier.uri	http://hdl.handle.net/1853/26256
dc.language.iso	en_US	en
dc.publisher	Georgia Institute of Technology	en
dc.subject	TCBS
dc.subject	vpa1263
dc.subject	Lateral Gene Transfer
dc.subject	LGT
dc.subject	Type III Secretion System
dc.subject	T3SS
dc.subject	Bacteria
dc.subject	Bacteriocin
dc.title	Lateral gene transfer of pandemic Vibrio parahaemolyticus genomic island genes	en
dc.type	Text
dc.type.genre	Undergraduate Thesis
dspace.entity.type	Publication
local.contributor.corporatename	College of Sciences
local.contributor.corporatename	School of Biological Sciences
local.contributor.corporatename	Undergraduate Research Opportunities Program
local.relation.ispartofseries	Undergraduate Research Option Theses
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