Probing Heme Trafficking Factors via Organellar Contact Points Using Genetically Encoded Fluorescent Heme Sensors

dc.contributor.advisor Reddi, Amit R.
dc.contributor.author Saini, Arushi
dc.contributor.committeeMember Oyelere , Adegboyega
dc.contributor.committeeMember Wilkinson, Angus
dc.contributor.department Chemistry and Biochemistry
dc.contributor.department Chemistry and Biochemistry
dc.date.accessioned 2020-11-09T16:58:31Z
dc.date.available 2020-11-09T16:58:31Z
dc.date.created 2019-05
dc.date.issued 2019-05
dc.date.submitted May 2019
dc.date.updated 2020-11-09T16:58:31Z
dc.description.abstract Heme is an important protein cofactor and signaling molecule that plays diverse roles in biological systems. The hydrophobicity and cytotoxicity of heme necessitates that it is transported and trafficked in a regulated manner. However, the molecules and mechanisms responsible for mediating heme trafficking remain poorly understood. Until recently, the tools to study heme in vivo did not exist, but the emergence of genetically encoded fluorescent sensors has enabled comprehensive real time analysis of heme in model organisms such as Saccharomyces cerevisiae. This study showcases a new a protocol that allows investigation of heme trafficking from its site of synthesis in the matrix side of the mitochondrial inner membrane to the outer matrix, cytosol, and nucleus over time. The method allows for the simultaneous examination of heme re-population in three cellular compartments after chemically depleting it. The study revealed that mitochondrial contact points play central roles in regulating heme availability and illuminates novel approaches to heme trafficking. These methods have the potential to be adapted to more inclusive compartmental analyses and enable a better understanding of heme trafficking which can empower innovative approaches to study infectious diseases, neurodegenerative disorders, and anemias associated with perturbations in heme cellular dynamics.
dc.description.degree Undergraduate
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/63831
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject Heme
dc.subject Fluorescent sensors
dc.subject Yeast
dc.subject Kinetics
dc.subject Saccharomyces cerevisiae
dc.subject Neurodegenerative disorders
dc.subject Mobilization
dc.subject Iron
dc.subject Contact points
dc.subject Anemia
dc.subject heme availability
dc.subject Cytosol
dc.subject Genetically encoded sensors
dc.subject Fluorescence
dc.subject Real time trafficking
dc.title Probing Heme Trafficking Factors via Organellar Contact Points Using Genetically Encoded Fluorescent Heme Sensors
dc.type Text
dc.type.genre Undergraduate Thesis
dspace.entity.type Publication
local.contributor.corporatename School of Chemistry and Biochemistry
local.contributor.corporatename College of Sciences
local.contributor.corporatename Undergraduate Research Opportunities Program
local.relation.ispartofseries Undergraduate Research Option Theses
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relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
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thesis.degree.level Undergraduate
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