The Mosaic Genome of Anaeromyxobacter dehalogenans Strain 2CP-C Suggests an Aerobic Common Ancestor to the Delta-Proteobacteria

dc.contributor.author Thomas, Sara H.
dc.contributor.author Wagner, Ryan D.
dc.contributor.author Arakaki, Adrian K.
dc.contributor.author Skolnick, Jeffrey
dc.contributor.author Kirby, John R.
dc.contributor.author Shimkets, Lawrence J.
dc.contributor.author Sanford, Robert A.
dc.contributor.author Löffler, Frank E.
dc.contributor.corporatename Georgia Institute of Technology. Center for the Study of Systems Biology
dc.contributor.corporatename Georgia Institute of Technology. School of Civil and Environmental Engineering
dc.contributor.corporatename University of Illinois at Urbana-Champaign. Dept. of Geology
dc.contributor.corporatename Georgia Institute of Technology. School of Biology
dc.contributor.corporatename University of Iowa. Dept. of Microbiology
dc.contributor.corporatename University of Georgia. Dept. of Microbiology
dc.date.accessioned 2009-01-30T18:11:41Z
dc.date.available 2009-01-30T18:11:41Z
dc.date.issued 2008-05-07
dc.description ©2008 Thomas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.description doi:10.1371/journal.pone.0002103
dc.description.abstract Anaeromyxobacter dehalogenans strain 2CP-C is a versaphilic delta-Proteobacterium distributed throughout many diverse soil and sediment environments. 16S rRNA gene phylogenetic analysis groups A. dehalogenans together with the myxobacteria, which have distinguishing characteristics including strictly aerobic metabolism, sporulation, fruiting body formation, and surface motility. Analysis of the 5.01 Mb strain 2CP-C genome substantiated that this organism is a myxobacterium but shares genotypic traits with the anaerobic majority of the delta-Proteobacteria (i.e., the Desulfuromonadales). Reflective of its respiratory versatility, strain 2CP-C possesses 68 genes coding for putative c-type cytochromes, including one gene with 40 heme binding motifs. Consistent with its relatedness to the myxobacteria, surface motility was observed in strain 2CP-C and multiple types of motility genes are present, including 28 genes for gliding, adventurous (A-) motility and 17 genes for type IV pilus-based motility (i.e., social (S-) motility) that all have homologs in Myxococcus xanthus. Although A. dehalogenans shares many metabolic traits with the anaerobic majority of the delta- Proteobacteria, strain 2CP-C grows under microaerophilic conditions and possesses detoxification systems for reactive oxygen species. Accordingly, two gene clusters coding for NADH dehydrogenase subunits and two cytochrome oxidase gene clusters in strain 2CP-C are similar to those in M. xanthus. Remarkably, strain 2CP-C possesses a third NADH dehydrogenase gene cluster and a cytochrome cbb3 oxidase gene cluster, apparently acquired through ancient horizontal gene transfer from a strictly anaerobic green sulfur bacterium. The mosaic nature of the A. dehalogenans strain 2CP-C genome suggests that the metabolically versatile, anaerobic members of the delta-Proteobacteria may have descended from aerobic ancestors with complex lifestyles. en
dc.identifier.citation PLoS ONE 3(5): e2103 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/1853/26883
dc.language.iso en_US en
dc.publisher Georgia Institute of Technology en
dc.publisher.original Institute of Electrical and Electronics Engineers, Inc., New York
dc.subject Genome sequencing en
dc.subject Anaeromyxobacter dehalogenans en
dc.subject Proteobacteria
dc.subject Phylogenetic and phenetic analyses
dc.title The Mosaic Genome of Anaeromyxobacter dehalogenans Strain 2CP-C Suggests an Aerobic Common Ancestor to the Delta-Proteobacteria en
dc.type Text
dc.type.genre Article
dspace.entity.type Publication
local.contributor.author Skolnick, Jeffrey
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
local.contributor.corporatename Center for the Study of Systems Biology
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