Title:
Mechanical Stretch and Electrical Stimulation in Mouse Skeletal Muscle in Vivo: Initiation of Hypertrophic Signaling

Simple item page
dc.contributor.advisor Burkholder, Thomas
dc.contributor.author Brathwaite, Ricky Christopher en_US
dc.contributor.committeeMember Cheng Zhu
dc.contributor.committeeMember Grace Pavlath
dc.contributor.department Bioengineering en_US
dc.date.accessioned 2005-03-02T22:36:13Z
dc.date.available 2005-03-02T22:36:13Z
dc.date.issued 2004-07-12 en_US
dc.description.abstract Skeletal muscle has an integral role in many activities. Although mechanical stretch and active force generation are known to be required for the maintenance of healthy muscle function, the mechanism by which those signals mediate muscle growth is unknown. This project was based on the hypothesis that stretch and force generation activate the Calcineurin/NFAT pathway and induce Cox-2 expression and initiate muscle hypertrophy. The specific aims of this study were to 1) develop a minimally invasive system capable of initiating hypertrophic signaling in mice, 2) characterize the effects of isometric activation, passive lengthening, and active lengthening on signaling cascades, and 3) determine the involvement of the Calcineurin/NFAT pathway and activation of COX-2 gene expression. We propose a pathway in which stimuli increase intracellular calcium, which activates the phosphatase calcineurin. Calcineurin dephosphorylates NFAT, which is translocated into the nucleus and initiates transcription of the COX-2 gene. COX-2 mediated synthesis of PGG2 is the rate-limiting step in bioactive prostaglandin synthesis. Prostaglandins then stimulate known hypertrophic signals including the PI-3 Kinase and MAP Kinase signaling cascades. en_US
dc.description.degree M.S. en_US
dc.format.extent 1613655 bytes
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/5074
dc.language.iso en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject PI3 Kinase en_US
dc.subject MAP Kinases
dc.subject NFAT
dc.subject Skeletal muscle
dc.subject COX-2
dc.subject Exercise
dc.subject Stimulation
dc.subject Stretch
dc.subject Mouse
dc.title Mechanical Stretch and Electrical Stimulation in Mouse Skeletal Muscle in Vivo: Initiation of Hypertrophic Signaling en_US
dc.type Text
dc.type.genre Thesis
dspace.entity.type Publication
local.contributor.corporatename College of Engineering
local.contributor.corporatename College of Engineering
local.relation.ispartofseries Master of Science in Bioengineering
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
relation.isSeriesOfPublication a5c9fe8c-5d03-4de6-a761-459fb2af0dea
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Brathwaite_Ricky_C_200407_MS.pdf
Size:
1.54 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Theses and Dissertations