Title:
Tools for Maximizing the Efficiency of Protein Engineering

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dc.contributor.advisor Bommarius, Andreas S.
dc.contributor.author Polizzi, Karen Marie en_US
dc.contributor.department Chemical Engineering en_US
dc.date.accessioned 2006-01-18T22:18:06Z
dc.date.available 2006-01-18T22:18:06Z
dc.date.issued 2005-11-14 en_US
dc.description.abstract Biocatalysts offer advantages over their chemical counterparts in terms of their high enantioselectivity and the opportunity to develop more environmentally friendly processes. However, the widespread adoption of biocatalytic processes is hampered by the long development times for enzymes with novel and sufficient activity and adequate stability under operating conditions. Protein engineering, while extremely useful for modifying the properties of protein catalysts in select cases, still cannot be performed rapidly enough for many applications. In order for biocatalysts to become a competitive alternative to chemical catalysts, new tools to make the tailoring of biocatalysts by protein engineering methods speedier and more efficient are necessary. The aim of this work was to develop methods to aid in the faster production of novel biocatalysts. Protein engineering involves two steps: the generation of diversity and the screening or selection of variants with the desired properties. Both of these must be targeted to create a faster protein engineering process. In the case of the former, this work sought to clone and overexpress some template enzymes which would create smaller, more manageable libraries of mutants with a higher likelihood of function by the manipulation of a few focused amino acid residues. For the latter, this work developed and validated a Monte-Carlo simulation model of pooling to increase screening throughput and created a set of vectors to aid in high-throughput screening by eliminating unwanted mutants from the assay procedure entirely. en_US
dc.description.degree Ph.D. en_US
dc.format.extent 1513500 bytes
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/7511
dc.language.iso en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Pooling en_US
dc.subject Cloning vector
dc.subject High-throughput screening
dc.subject Protein engineering
dc.subject Biocatalysis
dc.subject.lcsh Enzymes en_US
dc.subject.lcsh Protein engineering en_US
dc.subject.lcsh Biochemical engineering en_US
dc.title Tools for Maximizing the Efficiency of Protein Engineering en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Bommarius, Andreas S.
local.contributor.corporatename School of Chemical and Biomolecular Engineering
local.contributor.corporatename College of Engineering
relation.isAdvisorOfPublication faf2f937-2845-4a43-af4c-3d34a45ece32
relation.isOrgUnitOfPublication 6cfa2dc6-c5bf-4f6b-99a2-57105d8f7a6f
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
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