Title:
The development, characterization, and application of a biomimetic method of enzyme immobilization

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dc.contributor.advisor Kröger, Nils
dc.contributor.author Haase, Nicholas Rudy en_US
dc.contributor.committeeMember Doyle, Donald
dc.contributor.committeeMember Johnson, Glenn
dc.contributor.committeeMember Oyelere, Adegboyega
dc.contributor.committeeMember Sandhage, Kenneth
dc.contributor.department Chemistry and Biochemistry en_US
dc.date.accessioned 2013-01-17T21:19:41Z
dc.date.available 2013-01-17T21:19:41Z
dc.date.issued 2012-08-24 en_US
dc.description.abstract This dissertation describes the characterization of layer-by-layer silica and titania coatings deposited using a protamine-induced method. It was found that silica coatings were thinner and more porous than titania coatings. These coatings were functionalized by immobilizing modified Glucose oxidase during the layer-by-layer buildup. The enzyme was found to retain higher activity in silica versus titania, with full retention of activity observed in one configuration. Immobilization in both materials resulted in enhanced thermal stability and proteolytic protection. The enzyme-functionalized coatings were then modified by the immobilization of silver nanoparticles to the exterior, and this biological/inorganic composite was tested for its antimicrobial activity against E. coli and S. aureus. Against E. coli the composite worked in a synergistic fashion, showing more potent antimicrobial activity when compared to either agent used alone. The enzyme modification method was then extended to Laccase, which was immobilized to carbon nanotubes and characterized as a biocathode. Modified laccase returned a nearly two-fold higher current density versus the native enzyme. Finally, synthetic peptides were tested for their ability to adsorb to silica and titanium-oxide surfaces and subsequently deposit titanium-oxide coatings, in an effort to better understand the structure-function relationships of mineralizing peptides. en_US
dc.description.degree PhD en_US
dc.identifier.uri http://hdl.handle.net/1853/45802
dc.publisher Georgia Institute of Technology en_US
dc.subject Enzyme immobilization en_US
dc.subject Functional coatings en_US
dc.subject Biomimetics en_US
dc.subject.lcsh Biomimetics
dc.title The development, characterization, and application of a biomimetic method of enzyme immobilization en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.corporatename School of Chemistry and Biochemistry
local.contributor.corporatename College of Sciences
relation.isOrgUnitOfPublication f1725b93-3ab8-4c47-a4c3-3596c03d6f1e
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
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