Novel pathway for microbial FE(III) reduction: electron shuttling through naturally occurring thiols

dc.contributor.advisor DiChristina, Thomas J.
dc.contributor.author Wee, Seng Kew
dc.contributor.committeeMember Taillefert, Martial
dc.contributor.committeeMember Wartell, Roger M.
dc.contributor.committeeMember Hammer, Brian
dc.contributor.committeeMember Ingall, Ellery
dc.contributor.department Biology
dc.date.accessioned 2015-06-08T18:10:12Z
dc.date.available 2015-06-09T05:30:06Z
dc.date.created 2014-05
dc.date.issued 2014-02-17
dc.date.submitted May 2014
dc.date.updated 2015-06-08T18:10:12Z
dc.description.abstract The g-proteobacterium Shewanella oneidensis MR-1 reduces a wide range of terminal electron acceptors, including solid Fe(III) oxides. Pathways for Fe(III) oxide reduction by S. oneidensis include non-reductive (organic ligand-promoted) solubilization reactions, and either direct enzymatic, or indirect electron shuttling pathways. Results of the present study expand the spectrum of electron acceptors reduced by S. oneidensis to include the naturally occurring disulfide compounds cystine, oxidized glutathione, dithiodiglycolate, dithoidiproponiate and cystamine. Subsequent electron shuttling experiments demonstrated that S. oneidensis employs the reduced (thiol) form of the disulfide compounds (cysteine, reduced glutathione, mercaptoacetate, mercaptopropionate, and 2-nitro-5-thiobenzoate, cystamine) as electron shuttles to transfer electrons to extracellular Fe(III) oxides. The results of the present study indicate that microbial disulfide reduction may represent an important electron-shuttling pathway for electron transfer to Fe(III) oxides in anaerobic marine and freshwater environments.
dc.description.degree Ph.D.
dc.embargo.terms 2015-05-01
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/53406
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject Microbial Fe(III) reduction
dc.subject Thiols
dc.subject Shewanella
dc.title Novel pathway for microbial FE(III) reduction: electron shuttling through naturally occurring thiols
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor DiChristina, Thomas J.
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
relation.isAdvisorOfPublication cb6665c2-bcd0-4164-b448-e7ff33dd279f
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
relation.isOrgUnitOfPublication c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5
thesis.degree.level Doctoral
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