Wireless Biosensor System Electronics For Real-Time Monitoring of Bioreactor Cell Culture Properties
Author(s)
Rafay, Mohammed
Advisor(s)
Brown, Billyde
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Abstract
This thesis describes the development of the electronics for a miniaturized and portable wireless sensor system (i.e. Capsule) designed to replicate the functionality of large benchtop potentiostats. The overarching goal that extends beyond the scope of this project is to develop a wireless sensor capsule to detect and monitor critical quality attributes while inside a bioreactor in real-time during a human mesenchymal stem cell expansion process for tissue engineering applications. This thesis focuses on the design of the data acquisition and transceiver electronics and encompasses other aspects of the overarching project including the integration with a multiplexed sensor chip, and the design of a 3D-printed sterilizable packaging for the electronics. A key aspect of this project is the miniaturization of the electronics through a 3-phase development cycle that begins with the verification of the functional requirements of the wireless sensor capsule and ends with a product that can be used to test cell culture inside of a vertical wheel bioreactor. The end-product is capable of performing several electrochemical characterization techniques including potentiometry, chronoamperometry, and cyclic voltammetry and wirelessly providing sensor results to a smartphone or tablet in real-time using Bluetooth communication. The results from this capsule are shown to be comparable to large and accurate benchtop potentiostats such as the Keithley 4200A-SCS while being provided by a much smaller and portable device, ideal for point-of-care or point-of-production applications.
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Date
2022-05-03
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Thesis