Multimodal Electronic Cell Phenotyping via Multiplexed Impedance Sensors
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Arifuzzman, A. K. M.
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Abstract
Cell phenotyping, vital for understanding cellular behavior, employs mechanotyping, immunophenotyping, and apoptosis assays. In this thesis, we introduce three microfluidic platforms for advanced cell phenotyping. These platforms, tailored for clinical settings, cell manufacturing, and resource-limited environments, offer novel solutions. The first platform, an electronic cytometer microchip, facilitates high-throughput cell mechanotyping through Coulter-based snapshot sensors and polymer-based fluidic channels. It measures biomechanics, deriving quantitative viscoelastic properties. The second platform performs immunoanalysis on a portable, low-cost microchip, coupled with supporting hardware and software to operate autonomously. It adeptly processes unlabeled immune cells, streamlining the immunophenotyping process and positioning itself as an advanced tool for efficient, field-deployed analysis. The third platform, an Annexin V-based apoptosis assay, integrates an electrical sensor network with a multi-stage microfluidic biochip. It captures cells based on phosphatidylserine (PS) externalization, eliminating the need for pre-labeled samples. These microfluidic platforms revolutionize on-demand cell phenotyping, delivering real-time measurements and unlocking new capabilities in cellular research and diagnostics.
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Date
2023-12-12
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Dissertation