Engineering Nucleic Acid Nanostructures for Sensing and Control of Biomolecules and Their Environments
Author(s)
Pan, Victor
Advisor(s)
Lu, Zuhong
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Abstract
Nucleic acid nanotechnology has emerged in the past decade as a powerful and
versatile method to create functional and programmable nanostructures through
molecular self-assembly. The research presented in this dissertation uses a specific
nucleic acid assembly technique known as DNA Origami to create and apply designer
DNA nanostructures to three separate biotechnology-related applications.
The first is a rigid fluorescent barcode system utilizing hierarchically assembled DNA
origami nanorods, for use in situ labelling and detection of ssDNA and various proteins.
Second, DNA origami is used to control the distance between two synergistic but non
interacting proteins to study their cooperative binding to a third protein. Finally, an
adaptation of DNA origami is used to create a shear flow sensor capable of producing
fluorescent signal in response to fluidic shear.
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Date
2020-05-05
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Dissertation