Synthetic Antigen Binders: Novel applications for Structural and Cell Biology

Files
kossiakoff.mp4 (152.56 MB)
kossiakoff_videostream.html (985 B)
transcription.txt (42.27 KB)
Author(s)
Kossiakoff, Anthony A.
Advisor(s)
Editor(s)
Associated Organization(s)
Organizational Unit
Organizational Unit
School of Biological Sciences
School established in 2016 with the merger of the Schools of Applied Physiology and Biology
Organizational Unit
Series
Series
Collections
Scholarly Events
Supplementary to:
Permanent Link
http://hdl.handle.net/1853/36947
Abstract
Synthetic Antigen Binders (sABs) are a new class of antibody-based reagents engineered using novel phage display libraries. Using the power of phage display selection, it is possible generate sABs that : 1) target specific regions on the surface of a protein, 2) recognize specific conformational or oligomeric states, 3) induce conformational changes, and 4) capture and stabilize multi-protein complexes. Using these attributes, we can generate sABs that have their own functional properties and when introduced into cells can alter biological processes in defined ways. We have also employed them as crystallization chaperones to capture proteins in their most relevant states. As an example, the structure of the full-length KcsA potassium channel in both its closed and open states will be discussed. Further, we have also developed a cell delivery system that provides for introducing fully functional sABs into the cytoplasm for live cell imaging applications and to selectively alter cellular function.
Sponsor
Date
2011-02-08
Extent
57:50 minutes
Resource Type
Moving Image
Resource Subtype
Lecture
Rights Statement
Rights URI