Title:
Self–Assembled DNA Liquids: Properties and Protein Activation
Self–Assembled DNA Liquids: Properties and Protein Activation
No Thumbnail Available
Author(s)
Saleh, Omar
Advisor(s)
Editor(s)
Collections
Supplementary to
Permanent Link
Abstract
Biomolecules can self-assemble into liquid phases, termed ‘membraneless organelles’ in the biological context, though also known as ‘coacervates’. I will discuss our efforts to study this by exploiting DNA nanotechnology to create DNA particles that phase separate into liquids. Formation of liquids, rather than gel aggregates, depends sensitively on the internal flexibility of the DNA particles. Our engineered system displays unusual properties, including the ability to create several distinct liquid phases in a single solution, and to tailor interactions between the phases. Further, the reduced valency of the particles, along with the relatively stiff nature of the constituent DNA strands, causes the liquid to be extraordinarily sparse, with a DNA volume fraction of only ~2%. This opens the possibility to activate the material by infusion of the liquid with proteins; I will discuss our initial attempts at doing this.
Sponsor
Georgia Institute of Technology. College of Sciences
Georgia Institute of Technology. Institute for Materials
Georgia Institute of Technology. Parker H. Petit Institute for Bioengineering and Bioscience
Georgia Institute of Technology. School of Materials Science and Engineering
Georgia Institute of Technology. School of Physics
American Physical Society
Exxon Mobil Corporation
National Science Foundation (U.S.)
Georgia Institute of Technology. Institute for Materials
Georgia Institute of Technology. Parker H. Petit Institute for Bioengineering and Bioscience
Georgia Institute of Technology. School of Materials Science and Engineering
Georgia Institute of Technology. School of Physics
American Physical Society
Exxon Mobil Corporation
National Science Foundation (U.S.)
Date Issued
2018-04-19
Extent
42:04 minutes
Resource Type
Moving Image
Resource Subtype
Lecture