Title:
Rapid Susceptibility Testing from Blood-Derived Samples
Rapid Susceptibility Testing from Blood-Derived Samples
Author(s)
Filbrun, Alexandra
Advisor(s)
Dickson, Robert M.
Editor(s)
Collections
Supplementary to
Permanent Link
Abstract
Bacterial bloodstream infections contribute to global mortality and morbidity in large part due to the rapid development of antimicrobial resistance. Constrained to bacterial burdens of 1-10 colony-forming-units of bacteria per milliliter of blood (~109 blood cells), conventional diagnosis relies on lengthy culture-amplification and -isolation prior to identification and antibiotic susceptibility testing (ASTs). The resulting >60-hour time-to-result is at odds with the only indicator of survival – time to initiation of appropriate treatment. Consequently, new technologies capable of rapidly establishing actionable treatment from blood-derived samples is clinically important to promote positive patient outcomes and stewardship of antimicrobial resistance. To address this need, we developed a novel separation technology based on selective hemolysis coupled with centrifugation through inverted tubes containing sucrose. Demonstrated on the top BSI-causing bacterial families of Enterobacteriaceae (E. coli), non-fermenting gram-negative rods (P. aeruginosia), and Staphylococcus (S. aureus), near-pure bacteria is recovered from positive blood culture media in ≤15 minutes with minimal user intervention. Antibiotic susceptibilities are readily established using our label-free, flow cytometric approach for results in as little as 5 hours, post blood culture positivity. Overall, this direct from positive blood cultures enables results more than one day sooner than conventional methods.
Sponsor
Date Issued
2021-07-28
Extent
Resource Type
Text
Resource Subtype
Dissertation