Title:
A study of protein dynamics and cofactor interactions in Photosystem I

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dc.contributor.advisor Barry, Bridgette A.
dc.contributor.author Bender, Shana Lynn en_US
dc.contributor.committeeMember Doyle, Donald
dc.contributor.committeeMember Kelly, Wendy
dc.contributor.committeeMember McCarty, Nael
dc.contributor.committeeMember Schimdt-Krey, Ingaborg
dc.contributor.department Chemistry and Biochemistry en_US
dc.date.accessioned 2009-01-22T15:37:12Z
dc.date.available 2009-01-22T15:37:12Z
dc.date.issued 2008-11-10 en_US
dc.description.abstract Previous research has underscored the importance of protein dynamics during light-induced electron transfer; however, specific interactions have not been well characterized. It is of particular importance to understand the role of protein dynamics and cofactor interactions in controlling electron transfer in oxygenic photosynthesis. These factors include hydrogen bonding, ð-stacking and electrostatic interactions. Reaction-induced FT-IR spectroscopy is sensitive to these interactions as well as isotopic incorporation, and is useful to probe protein dynamics associated with light-induced electron transfer in Photosystem I (PSI). Density functional theory (DFT) provides information concerning the vibrational frequencies of molecules as well as the amplitudes of the vibrations and sensitivity to isotope incorporation. Combining these approaches, protein dynamics associated with light-induced electron transfer in PSI were studied. The work presented here describes specific protein cofactor interactions and specific protein relaxation events associated with light-induced electron transfer. The results reported here are consistent with noncovalent protein cofactor interactions that modulate the redox potential of the secondary electron acceptor of PSI. Furthermore, the studies presented here describe novel protein dynamics associated with the oxidation of the terminal electron donor of PSI. These results characterize specific protein dynamics that may be associated with interactions of the soluble electron donors. These studies highlight the importance of protein dynamics in oxygenic photosynthesis. en_US
dc.description.degree Ph.D. en_US
dc.identifier.uri http://hdl.handle.net/1853/26463
dc.publisher Georgia Institute of Technology en_US
dc.subject FT-IR spectroscopy en_US
dc.subject Photosysystem I en_US
dc.subject Protein dynamics en_US
dc.subject Electron transfer en_US
dc.subject.lcsh Charge exchange
dc.subject.lcsh Photosynthesis
dc.subject.lcsh Proteins Structure-activity relationships
dc.title A study of protein dynamics and cofactor interactions in Photosystem I en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Barry, Bridgette A.
local.contributor.corporatename School of Chemistry and Biochemistry
local.contributor.corporatename College of Sciences
relation.isAdvisorOfPublication 3398b34c-4658-496b-84ec-43b16a2007e0
relation.isOrgUnitOfPublication f1725b93-3ab8-4c47-a4c3-3596c03d6f1e
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
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