Title:
Sustained Delivery of Thermally Stabilized chABC by Lipid Microtubules

dc.contributor.author Kumar, Nathan en_US
dc.contributor.department Biology en_US
dc.date.accessioned 2008-05-22T17:09:15Z
dc.date.available 2008-05-22T17:09:15Z
dc.date.issued 2008-05-05 en_US
dc.description.abstract Our knowledge of spinal cord injury repair is broadening with the developing technology for nerve regeneration and drug delivery. In this paper we discuss the current capabilities for spinal cord repair as well as those that are in development. We develop protocols for determining the thermal stability of chondroitinase ABCI and its ability to be implanted into a microtubule-hydrogel drug delivery vehicle as well as the release profile that results from this implantation. After the use of sodium dodecyl electrophoresis, we determined that the disaccharide trehalose has the capacity to thermally stabilize our therapeutic enzyme in vitro. We also determined that the microtubules are effective for sustaining the release of our enzyme while the hydrogel is effective for localizing its effects. The deactivation profile was experimentally quantified to allow for complete diffusion of our enzyme over the course of a two-week implantation. Our thermally stabilized enzyme and drug delivery system can be used for the purpose of facilitating nerve regeneration at the site of an injury. en_US
dc.description.advisor Faculty Mentor: Ravi Bellamkonda en_US
dc.identifier.uri http://hdl.handle.net/1853/21833
dc.publisher Georgia Institute of Technology en_US
dc.subject Chondroitinase ABC en_US
dc.subject Microtubule en_US
dc.title Sustained Delivery of Thermally Stabilized chABC by Lipid Microtubules en_US
dc.type Text
dc.type.genre Undergraduate Thesis
dspace.entity.type Publication
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
local.contributor.corporatename Undergraduate Research Opportunities Program
local.relation.ispartofseries Undergraduate Research Option Theses
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
relation.isOrgUnitOfPublication c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5
relation.isOrgUnitOfPublication 0db885f5-939b-4de1-807b-f2ec73714200
relation.isSeriesOfPublication e1a827bd-cf25-4b83-ba24-70848b7036ac
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Final_Thesis_5_1_2008c.pdf
Size:
674.7 KB
Format:
Adobe Portable Document Format
Description: