Title:
TP508 maintains chondrocyte cell viability through blocking apoptosis in
an NO-dependent manner
TP508 maintains chondrocyte cell viability through blocking apoptosis in
an NO-dependent manner
Author(s)
Zhong, Ming
Advisor(s)
Boyan, Barbara D.
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Abstract
TP508 is a 23 amino acid peptide derived from human prothrombin. It helps
wound healing in both soft tissues and bones. In our previous study, we have
demonstrated that TP508 retains chondrocyte in a less mature differentiation
state while expanding the cartilage mass, indicating it may partly help bone
healing by expand the cartilage template in the endochondral bone formation
stage. In our current study, we want to demonstrate that TP508 also blocks
chondrocyte apoptosis. We used rat costochondral growth plate chondrocytes
as our model. We first established chelerythrine as an apoptogen in
chondrocytes. TP508 is able to block apoptosis caused by chelerythrine.
Chelerythrine also causes an increase in NO production, which is known to
cause both pathological and physiological apoptosis of chondrocyte, and
blocking NO production can in turn block apoptosis caused by them. TP508 is
also able to block NO production caused by chelerythrine. Therefore, TP508
may partially block chondrocyte apoptosis by blocking NO production. From all above,
we conclude that besides decreasing chondrocyte differentiation, TP508 also
blocks their apoptosis, so as to conserve the cartilage template in
endochondral bone formation
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Date Issued
2006-11-27
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