Title:
Systems and Methods Enabling Patch-Clamp Re-Use
Systems and Methods Enabling Patch-Clamp Re-Use
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Abstract
A method for cleaning patch-clamp glass pipette electrodes that enables their re-use. By immersing pipette tips or planar patch clamp chips into a detergent, followed by rinsing, pipettes and planar patch clamp chips were re-usable at least ten times with little to no degradation in signal fidelity, in experimental preparations ranging from human embryonic kidney cells to neurons in culture, slices, and in vivo.
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11/10/2020