Title:
Wavy-Walled Bioreactor Supports Increased Cell Proliferation and Matrix Deposition in Engineered Cartilage Constructs

dc.contributor.author Bueno, Ericka M. en_US
dc.contributor.author Bilgen, Bahar en_US
dc.contributor.author Barabino, Gilda A. en_US
dc.contributor.corporatename Northeastern University. Dept. of Chemical Engineering en_US
dc.date.accessioned 2013-06-12T20:22:57Z
dc.date.available 2013-06-12T20:22:57Z
dc.date.issued 2005-11
dc.description This is a copy of an article published in Tissue Engineering © 2005 Mary Ann Liebert, Inc.; Tissue Engineering is available online at: http://online.liebertpub.com en_US
dc.description DOI: 10.1089/ten.2005.11.1699 en_US
dc.description.abstract Hydrodynamic forces in bioreactors can decisively influence extracellular matrix deposition in engineered cartilage constructs. In the present study, the reduced fluid shear, high-axial mixing environment provided by a wavy-walled bioreactor was exploited in the cultivation of cartilage constructs using polyglycolic acid scaffolds seeded with bovine articular chondrocytes. Increased growth as defined by weight, cell proliferation and extracellular matrix deposition was observed in cartilage constructs from wavy-walled bioreactors in comparison with those from spinner flasks cultured under the same conditions. The wet weight composition of 4-week constructs from the wavy-walled bioreactor was similar to that of spinner flask constructs, but the former were 60% heavier due to equally higher incorporation of extracellular matrix and 30% higher cell population. It is most likely that increased construct matrix incorporation was a result of increased mitotic activity of chondrocytes cultured in the environment of the wavy-walled bioreactor. A layer of elongated cells embedded in type I collagen formed at the periphery of wavy-walled bioreactor and spinner flask constructs, possibly as a response to local shear forces. On the basis of the robustness and reproducibility of the extracellular matrix composition of cartilage constructs, the wavy-walled bioreactor demonstrated promise as an experimental cartilage tissue-engineering vessel. Increased construct growth in the wavy-walled bioreactor may lead to enhanced mechanical properties and expedited in vitro cultivation. en_US
dc.identifier.citation Ericka M. Bueno, Bahar Bilgen, and Gilda A. Barabino, "Wavy-Walled Bioreactor Supports Increased Cell Proliferation and Matrix Deposition in Engineered Cartilage Constructs," Tissue Engineering, 11, 11-12, 1699-1709 (November/December 2005) en_US
dc.identifier.doi 10.1089/ten.2005.11.1699
dc.identifier.issn 1076-3279
dc.identifier.uri http://hdl.handle.net/1853/47404
dc.language.iso en_US en_US
dc.publisher Georgia Institute of Technology en_US
dc.publisher.original Mary Ann Liebert, Inc. en_US
dc.subject Bioreactors en_US
dc.subject Extracellular matrix deposition en_US
dc.subject Wavy-walled bioreactor en_US
dc.subject Cell proliferation en_US
dc.title Wavy-Walled Bioreactor Supports Increased Cell Proliferation and Matrix Deposition in Engineered Cartilage Constructs en_US
dc.title.alternative Increased matrix production in engineered cartilage constructs cultivated in a wavy-walled bioreactor en_US
dc.type Text
dc.type.genre Article
dspace.entity.type Publication
local.contributor.corporatename Wallace H. Coulter Department of Biomedical Engineering
local.contributor.corporatename College of Engineering
relation.isOrgUnitOfPublication da59be3c-3d0a-41da-91b9-ebe2ecc83b66
relation.isOrgUnitOfPublication 7c022d60-21d5-497c-b552-95e489a06569
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