Exploring Morphological Changes and Cell Activity with Cell Number Variation in Organoids: Implications for Cancer Invasion Studies
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Sim, Abigail Rachelle
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Organoid models represent a powerful tool for investigating complex cellular behaviors and disease processes in a controlled, three-dimensional environment. In this thesis, we explore the dynamic interplay between cellular activity, structural integrity, and cancer invasion potential within MCF10A organoids. Through a combination of cell culture, fluorescence microscopy, and mechanical testing, we discuss the effects of varying cell concentrations on organoid behavior and function. Our results reveal distinct patterns of ATP activity and tissue surface tension (TST) across different cell concentrations, highlighting the intricate relationship between cellular metabolism and mechanical properties within the organoids. Fluorescence microscopy imaging further uncovers structural and molecular differences between organoid conditions, providing valuable insights into cellular organization and cytoskeletal dynamics. These findings shed light on the implications of these observations for cancer invasion studies, demonstrating how variations in cell concentration may influence tumor behavior and response to therapy within organoid models. By unraveling the complexities of organoid biology, this research contributes to our understanding of cellular dynamics and disease processes, paving the way for new insights into cancer biology and therapeutic development. Overall, this thesis represents a comprehensive investigation into the multifaceted nature of MCF10A organoids and their relevance for understanding cellular behavior in health and disease. Through interdisciplinary approaches, we provide novel insights into organoid biology and its implications for cancer research and therapeutic interventions.
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Undergraduate Research Option Thesis