Title:
Discovery of Monoclonal Antibodies for Diagnostics and Therapies Using Conjugate Virus-like Particle Vaccines

dc.contributor.advisor Finn, M. G.
dc.contributor.author Schroeder, Michelle
dc.contributor.committeeMember Thomas, Susan
dc.contributor.department Chemistry and Biochemistry
dc.date.accessioned 2021-06-30T17:37:40Z
dc.date.available 2021-06-30T17:37:40Z
dc.date.created 2021-05
dc.date.issued 2021-05
dc.date.submitted May 2021
dc.date.updated 2021-06-30T17:37:40Z
dc.description.abstract Monoclonal antibodies (mAbs) are highly specific antigen binding proteins that are used as biological reagents, therapeutics, and in rapid diagnostics. While mAbs have extensive potential applications, their means production for small molecules and conformationally specific peptides is difficult. Here, we use a method of mAb production in which we pair conjugate virus-like particle (VLP) vaccine with hybridoma technology to produce high-affinity mAbs against three classes of molecules 1) fentanyl derivatives, 2) SARS-CoV-2 peptides, and 3) α-amanitin and microcystin LR cyclic peptide toxins. We successfully produced broad and derivative-selective mAbs against eight fentanyl derivatives. We also showed early signs of success targeting neutralizing and mutant SARS-CoV-2 peptides with conformational specificity using a heterologous prime-boost strategy. Lastly, we produced high affinity mAbs for both α-amanitin and microcystin LR, two highly toxic cyclic peptides. The early success of mAb production against the variety of targets presented in this thesis shows the viability and exceptional versatility of conjugate VLP vaccines as a means to producing mAbs.
dc.description.degree Undergraduate
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/64866
dc.language.iso en_US
dc.publisher Georgia Institute of Technology
dc.subject Immunology
dc.subject Vaccine
dc.subject Nanoparticles
dc.subject Antibodies
dc.title Discovery of Monoclonal Antibodies for Diagnostics and Therapies Using Conjugate Virus-like Particle Vaccines
dc.type Text
dc.type.genre Undergraduate Thesis
dspace.entity.type Publication
local.contributor.advisor Finn, M. G.
local.contributor.corporatename School of Chemistry and Biochemistry
local.contributor.corporatename College of Sciences
local.contributor.corporatename Undergraduate Research Opportunities Program
local.relation.ispartofseries Undergraduate Research Option Theses
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thesis.degree.level Undergraduate
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