Genetics under geothermal conditions: Homologous recombination in the archaeon Sulfolobus acidocaldarius

Grogan, Dennis

Title:

Genetics under geothermal conditions: Homologous recombination in the archaeon Sulfolobus acidocaldarius

dc.contributor.author	Grogan, Dennis
dc.contributor.corporatename	University of Cincinnati
dc.contributor.corporatename	Georgia Institute of Technology. School of Biology
dc.date.accessioned	2009-02-10T20:07:59Z
dc.date.available	2009-02-10T20:07:59Z
dc.date.issued	2008-10-23
dc.description	Presented on October 23, 2008 from 11:00 AM – 12:00 PM in room L1205, Ford Environmental Science & Technology Building (ES&T) on the Georgia Tech campus.	en
dc.description	Host: Center For Environmental Systems Microbiology (CESM)
dc.description	Runtime: 47:52 minutes
dc.description.abstract	Hyperthermophilic archaea differ radically from all model organisms with respect to their evolutionary history and the severe environmental conditions they require. This divergence raises questions as to whether their genetic processes also have unusual properties; but few of these processes have been analyzed in vivo. In the extreme thermoacidophile Sulfolobus acidocaldarius, a conjugational mechanism of DNA transfer enables recombination between chromosomal mutations to be quantified. Early studies of this system suggested a non-reciprocal mechanism in which donor sequences become incorporated into the recipient genome as short segments. Subsequent studies using electroporation found that synthetic oligonucleotides can recombine into this genome. When similar experiments used longer, duplex DNAs containing multiple, silent markers, the resulting recombinants often contained multiple replacement tracts, consistent with an unusual, "short-patch" mode of homologous recombination.	en
dc.format.extent	47:52 minutes
dc.identifier.uri	http://hdl.handle.net/1853/26935
dc.language.iso	en_US	en
dc.publisher	Georgia Institute of Technology	en
dc.subject	Archaea	en
dc.subject	Hyperthermophiles	en
dc.subject	Genetic processes	en
dc.subject	Homologous recombination	en
dc.title	Genetics under geothermal conditions: Homologous recombination in the archaeon Sulfolobus acidocaldarius	en
dc.type	Moving Image
dc.type.genre	Lecture
dspace.entity.type	Publication
local.contributor.corporatename	College of Sciences
local.contributor.corporatename	School of Biological Sciences
local.relation.ispartofseries	School of Biology Seminar Series
relation.isOrgUnitOfPublication	85042be6-2d68-4e07-b384-e1f908fae48a
relation.isOrgUnitOfPublication	c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5
relation.isSeriesOfPublication	146d0d36-ad75-4642-ad2a-96dcbf372f06