Title:
Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine

dc.contributor.advisor Pullman, Gerald S.
dc.contributor.author Jones, Brande en_US
dc.contributor.committeeMember Cairney, John
dc.contributor.committeeMember Chernoff, Yury
dc.contributor.committeeMember Choi, Jung
dc.contributor.committeeMember Dean, Jeffrey
dc.contributor.committeeMember Wartell, Roger
dc.contributor.department Biology en_US
dc.date.accessioned 2012-06-06T16:42:57Z
dc.date.available 2012-06-06T16:42:57Z
dc.date.issued 2011-01-22 en_US
dc.description.abstract A basic understanding of the molecular events occurring during zygotic embryogenesis is required to fully understand how and why only a very small percentage of somatic embryos develop past the late embryogeny phase of embryogenesis. In this work, we have identified genes that have been demonstrated to be required for late embryonic development in the model plant system Arabidopsis thaliana. These genes were subsequently isolated and cloned from Loblolly pine embryos. These isolated clones were sequenced and analyzed to reveal significant homology to the known Arabidopsis ABA responsive genes ABI3, ABI4, and ABI5. Expression analyses of all three genes were completed, and compared to reported data of ABA accumulation, as well as, expression of other ABA responsive genes during the same stages of embryogenesis. Six putative root development genes were isolated and cloned from Loblolly pine embryos. These isolated clones were sequenced and analyzed to reveal significant homology to the known Arabidopsis root development genes WOODENLEG, SHORT ROOT, SCARECROW, HOBBIT, BODENLOS, and MONOPTEROS. Full-length cDNAs were isolated and cloned for WOODENLEG, SHORT ROOT, SCARECROW and BODENLOS. Expression analyses of all six genes were completed throughout mid to late embryogenesis in Loblolly pine. en_US
dc.description.degree PhD en_US
dc.identifier.uri http://hdl.handle.net/1853/43598
dc.publisher Georgia Institute of Technology en_US
dc.subject Plant genetics en_US
dc.subject Somatic embryogenesis en_US
dc.subject Root development en_US
dc.subject.lcsh Arabidopsis
dc.subject.lcsh Arabidopsis thaliana
dc.subject.lcsh Loblolly pine
dc.subject.lcsh Roots (Botany) Development
dc.title Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine en_US
dc.type Text
dc.type.genre Dissertation
dspace.entity.type Publication
local.contributor.advisor Pullman, Gerald S.
local.contributor.corporatename College of Sciences
local.contributor.corporatename School of Biological Sciences
relation.isAdvisorOfPublication 2dcdf005-8488-45aa-9e85-7582bb6d7b51
relation.isOrgUnitOfPublication 85042be6-2d68-4e07-b384-e1f908fae48a
relation.isOrgUnitOfPublication c8b3bd08-9989-40d3-afe3-e0ad8d5c72b5
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