The Influence of Normal Physiological Forces on Porcine Aortic Heart Valves in a Sterile Ex Vivo Pulsatile Organ Culture System

dc.contributor.advisor Yoganathan, Ajit P.
dc.contributor.author Konduri, Suchitra en_US
dc.contributor.committeeMember Sambanis, Athanassios
dc.contributor.committeeMember Timothy M. Wick
dc.contributor.department Chemical Engineering en_US
dc.date.accessioned 2005-07-28T19:08:05Z
dc.date.available 2005-07-28T19:08:05Z
dc.date.issued 2005-03-17 en_US
dc.description.abstract The aortic valve functions in a complex mechanical environment which leads to force dependent cellular and tissue responses. Characterization of these responses provides a fundamental understanding of valve pathogenesis. The aim of this work was to develop an ex vivo organ culture system capable of simulating physiological aortic pressures and flow rates, and study the biological characteristics of native porcine aortic valves cultured in the system. Collagen, sGAG and elastin content of the valve leaflets were measured and cusp morphology, cell phenotype, cell proliferation and apoptosis were examined. Presence of endothelial cells (ECs) on the leaflet surface was also evaluated. The differences in collagen, sGAG and elastin contents were not significant (p greater than0.05) between the cultured and fresh valve leaflets. The cultured valves maintained the structural integrity of the leaflets while preserving the native morphology and cell phenotype. Cell phenotype in leaflets incubated statically under atmospheric conditions decreased compared to fresh and cultured valve leaflets, indicating the importance of mechanical forces in maintaining the natural biology of the valve leaflets. ECs were retained on the surfaces of cultured leaflets with no remodeling of the leaflets. The number of apoptotic cells in the cultured leaflets was significantly (p less than 0.05) less than in the statically incubated leaflets and comparable to fresh leaflets. The sterile ex vivo organ culture system thus maintained the viability and native biological characteristics of the aortic valves that were cultured under dynamic conditions for a period of 48 hours. en_US
dc.description.degree M.S. en_US
dc.format.extent 3204033 bytes
dc.format.mimetype application/pdf
dc.identifier.uri http://hdl.handle.net/1853/6999
dc.language.iso en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Flow and pressure waveforms en_US
dc.subject Porcine aortic valve leaflets
dc.subject Extracellular matrix components
dc.subject Cell phenotype
dc.subject Endothelial cells
dc.subject Organ culture system
dc.subject Tissue morphology
dc.title The Influence of Normal Physiological Forces on Porcine Aortic Heart Valves in a Sterile Ex Vivo Pulsatile Organ Culture System en_US
dc.type Text
dc.type.genre Thesis
dspace.entity.type Publication
local.contributor.advisor Yoganathan, Ajit P.
local.contributor.corporatename School of Chemical and Biomolecular Engineering
local.contributor.corporatename College of Engineering
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