Person:
Kubanek, Julia

Permanent Link
https://hdl.handle.net/1853/71428
Associated Organization(s)
Organizational Unit
School of Biological Sciences
School established in 2016 with the merger of the Schools of Applied Physiology and Biology
ORCID
ArchiveSpace Name Record

Filters

2012 - 2017 6
5 1
1
6
Reset filters

Settings
Has files: Yes × Start date: 2012 ×

Publication Search Results

Now showing 1 - 6 of 6
No Thumbnail Available
Item

Metabolomics and Proteomics Reveal Impacts of Chemically Mediated Competition on Marine Plankton Dataset

2017-12-22 , Poulson-Ellestad, Kelsey L. , Jones, Christina , Roy, Jessie , Viant, Mark , Fernández, Facundo M. , Kubanek, Julia , Nunn, Brook

Competition is a major force structuring marine planktonic communities. The release of compounds that inhibit competitors, a process known as allelopathy, may play a role in the maintenance of large blooms of the red-tide dinoflagellate Karenia brevis, which produces potent neurotoxins that negatively impact coastal marine ecosystems. K. brevis is variably allelopathic to multiple competitors, typically causing sublethal suppression of growth. We used metabolomic and proteomic analyses to investigate the role of chemically mediated ecological interactions between K. brevis and two diatom competitors, Asterionellopsis glacialis and Thalassiosira pseudonana. The impact of K. brevis allelopathy on competitor physiology was reflected in the metabolomes and expressed proteomes of both diatoms, although the diatom that co-occurs with K. brevis blooms (A. glacialis) exhibited more robust metabolism in response to K. brevis. The observed partial resistance of A. glacialis to allelopathy may be a result of its frequent exposure to K. brevis blooms in the Gulf of Mexico. For the more sensitive diatom, T. pseudonana, which may not have had opportunity to evolve resistance to K. brevis, allelopathy disrupted energy metabolism and impeded cellular protection mechanisms including altered cell membrane components, inhibited osmoregulation, and increased oxidative stress. Allelopathic compounds appear to target multiple physiological pathways in sensitive competitors, demonstrating that chemical cues in the plankton have the potential to alter large-scale ecosystem processes including primary production and nutrient cycling.

View
No Thumbnail Available
Item

Karenia brevis allelopathy compromises the lipidome, membrane integrity, and photosynthesis of competitors (dataset)

2015-12-17 , Poulin, R. X. , Kubanek, Julia

The attached data files underlie the forthcoming publication, "Karenia brevis allelopathy compromises the lipidome, membrane integrity, and photosynthesis of competitors".

View
No Thumbnail Available
Item

Chemical Encoding of Risk Perception and Predator Detection Among Estuarine Invertebrates Dataset

2017-12-07 , Poulin, Remington X. , Lavoie, Serge , Siegel, Katherine , Gaul, David A. , Weissburg, Marc J. , Kubanek, Julia

The data files in "Archived PCA Spectra" and “Archived PLS-R Spectra” contain the unprocessed 1H NMR spectral data files underlying the publication " Chemical encoding of risk perception and predator detection among estuarine invertebrates."

View
No Thumbnail Available
Item

You are what you eat: A combined metabolomics – bioassay approach to understanding prey responses to chemical cues produced by predators fed different diets dataset

2015-09-09 , Weissburg, Marc J. , Poulin, R.X. , Kubanek, Julia

View
No Thumbnail Available
Item

Variable Allelopathy Among Phytoplankton Reflected in Red Tide Metabolome Dataset

2017-12-06 , Poulin, Remington X. , Poulson-Ellestad, Kelsey L. , Roy, Jessie S. , Kubanek, Julia

Dataset for Harmful Algae Manuscript titled: "Variable allelopathy among phytoplankton reflected in red tide metabolome"

View
No Thumbnail Available
Item

Warding Off Disease on Coral Reefs: Antimicrobial Chemical Cues and their Future in Drug Discovery

2012-05-08 , Kubanek, Julia

Unlike many animals, seaweeds do not possess adaptive immune systems to ward off disease. In many cases, they produce small molecules – natural antibiotics – that prevent colonization or infection by pathogens. We have found that seaweeds produce unusual secondary metabolites against pathogen attack, including complex isoprenoid-and shikimate-derived macrolides not seen in any other organisms. Surprisingly, these natural antifungals are not distributed evenly across algal surfaces; instead they are concentrated at discrete surface patches where they provide bursts of protection at sites that may be especially vulnerable to infection due to prior wounding. Working with the Fernandez lab at Georgia Tech, we applied surface imaging mass spectrometry to intact algal surfaces to show that antifungal defenses are heterogeneously distributed, with compound concentrations high enough at localized patches to block infection. This patchy distribution may represent an optimal defense strategy, in which the most vulnerable parts of the alga are best defended. Chemical defenses of seaweeds also serve as valuable leads for pharmaceutical development. Members of one group of algal antifungal agents, the bromophycolides, exhibit potent in vitro and in vivo antimalarial activity with an unexpected mechanism of action, inhibiting growth of the malarial parasite Plasmodium falciparum at sub-micromolar concentrations. Using a molecular probe designed from the seaweed’s antifungal agent, we identified a major molecular target and drug binding mechanism within the malaria parasite that is helping guide our synthesis of novel analogs for future development.

View