Person:
Nie, Shuming

Permanent Link
https://hdl.handle.net/1853/72535
Associated Organization(s)
Organizational Unit
Wallace H. Coulter Department of Biomedical Engineering
The joint Georgia Tech and Emory department was established in 1997
ORCID
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    Cancer Nanotechnology
    (Georgia Institute of Technology, 2009-05-01) Nie, Shuming ; McDonald, John F. ; El-Sayed, Mostafa A.
    Shuming Nie is the Wallace H. Coulter Distinguished Chair Professor in Biomedical Engineering at Emory University and the Georgia Institute of Technology. His research interest is broadly in biomolecular engineering and nanotechnology. John McDonald is taking an integrated systems approach to the study of cancer. This means that he views cancer not as a defect in any particular gene or protein, but as a de-regulated cellular/ inter-cellular process. Mostafa El-Sayed is the Julius Brown Chair and Regents Professor in the School of Chemistry and Biochemistry at Georgia Tech. He researches Nanoscience and also investigates how Nanoparticles can be used in Nanomedicine, Nano Catalysis, and Nanophotonics.
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    Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells
    (Georgia Institute of Technology, 2004) Nitin, Nitin ; Santangelo, Philip J. ; Kim, Gloria ; Nie, Shuming ; Bao, Gang
    Real‐time visualization of specific endogenous mRNA expression in vivo has the potential to revolutionize medical diagnosis, drug discovery, developmental and molecular biology. However, conventional liposome‐ or dendrimer‐based cellular delivery of molecular probes is inefficient, slow, and often detrimental to the probes. Here we demonstrate the rapid and sensitive detection of RNA in living cells using peptide‐linked molecular beacons that possess self‐delivery, targeting and reporting functions. We conjugated the TAT peptide to molecular beacons using three different linkages and demonstrated that, at relatively low concentrations, these molecular beacon constructs were internalized into living cells within 30 min with nearly 100% efficiency. Further, peptide‐based delivery did not interfere with either specific targeting by or hybridization‐induced fluorescence of the probes. We could therefore detect human GAPDH and survivin mRNAs in living cells fluorescently, revealing intriguing intracellular localization patterns of mRNA. We clearly demonstrated that cellular delivery of molecular beacons using the peptide‐based approach has far better performance compared with conventional transfection methods. The peptide‐linked molecular beacons approach promises to open new and exciting opportunities in sensitive gene detection and quantification in vivo.