You know I'm right here.

Research cancer research might be faster

or their research really touches on

understanding how cells where

we are about these things.

Thank you for the current introduction.

Can you hear me in the back.

OK so my name is Tony came the topic.

I have today is engineering by
marriage my persistence for

another medicine for another Madison is
for us still leaving the translation.

Now on the medicines.

As we all know the medicine is just
defined there is it medical to occasional

technology many people who
are trying to create really so

few skated the particles which
has a certain encapsulated.

And targeting organs and imaging Egypt and

now they campaign multiple
imaging agents and

drugs within the novel
particles were inside core.

So we call this motif.

We've noticed targeting I go
What happen is once we inject or

ask the patient to have this
throws then you will spit out

the body as expected and
we will have a certain style.

Effect which is not really
important in most cases but

when you think about the cancer.

If you want to really kill the cancer
cells in your body you don't want to get

this side staring you
in through the belly.

So we want to make this
target a certain area

which all the cancer cells
are tumor cells around.

So the target is important as Cup Rob
strategist for example we can

use without targeting Lyons just
suspend on our policy in Europe

world stream and using this premier
ability because usually when you cancel

cells located your blood bells will
really really key and dysfunctional.

So we can utilize to situations and
just spend

another part of trying to keep those
nano particles in your bloodstream.

As long as possible so we can we can

transport those drugs to target cells
which is cancerous cells in this case and

sometimes if we your cells
really inflammation such as

color of a school of these years and
if you have a surgeon or her Couric clout.

Then you may want to treat
the best course of Anderson cells

themselves rather than target cells.

So you can treat.

And make the situation better on the area.

And in the last case you can
typically target the cells inside.

So in this example shows
that we need to target.

And we need is multiple
imaging Asian intros

to more neuter the Nano particle
we suspend in your body.

So we can think about the tongue Ryan
of the critical stage on the medicines.

Let me show you some representative.

Not a poly cause people develop libels
them since nine hundred sixty is

pretty long time ago day used forceful
it is standard nano particles which

is clearly because you have hydrophilic
surface which is stable in your burst.

Three mm.

But you have a certain look or

area between there is a little
smaller space for hydrophobia throws.

That's why people are trying to going to
make poor based on the particles which

is better in terms of
the hydrophobic throw building.

But this lighter germ is still available.

It's very unique you won't see you get
anti-bias falling properties such as

pagal ations to once you
get this pad outside or

weird right with them so you can you
can make is not a particle longer

save in your bloodstream and
you can also put the target in wires so

Doc still is the first at the approved
nano particles in our timeline and

you can also put a protein based or
a certain in or

going on a crystal or an outside and you
can also make this a little hybrid hybrid

type platform the polymer
either four split by a layer or

my cell in the poem or
invalid my stills and

now days not only just
prior to this thing go.

This after a certain type
of teen star your body.

You're using multiple targeting wagons.

And now an apologist is
going to very complicated.

Then what are the challenges
the challenges many people listen to

leave poor in relief.

How impact Jones like Chancellor
Merkel Madison's so many papers.

But still few drugs and a lot of
critics because when you look at this

graph listener Charles per year
last year we had up to thirty

thousand papers just depending
on the nano particles and

nano Madison with a certain drug and
imaging agents how we can I be.

To how many labs and
people are working on this and

around two hundred is applied and
so made it for pate.

And just hand is going critical triers

just trying to make sure this
tan is not in two thousand and

thirteen is until now some
of those rejected fail.

Still we have ten critical triers
some point to last year and

every year from now the medicine.

Maybe a few hopefully we can cope.

But or none in years.

So what are the challenges
these peoples pointed out is to

one is it's really hard to reproduce.

Once we synthesize certain
type of nano particles

because like I said another public
platform is getting complicated because

we need multiple amusing Asian
multiple multiple drug compounds.

So even though I said
the same companies ition.

I preparing on the bench in
same place same time tomorrow

you will get a different properties.

That's a frustrating and that's the one of
the top challenges we have right now and

it will be getting worse.

Once you try to scale this up.

Because why we need to scale it
up because what we need is and

you wait for own term we want
to use this for a human body.

How many how much drug we need for a human
body compared to how much we need for

just one simple the red or mouse
experiment is totally different things.

So the kids can really only get the high
reproducible particles even skill and

the other challenge is low success rate in
animals to this uncritical try yours with

her two hikers who you all know
how expensive this to this are and

once you develop and
hope to marginal particles and

make sure that disguise that good
enough in India to study in your.

Dish or any cells of your
culture in the sheaf Berman.

And then once you test it anymore.

The small.

You all.

Most of the cast is the now apologise to
fail and because that's the gap between

our in between barman and
even the small animal status.

So let me show you the typical
translation steps of normal medicine

clinical development.

So it takes you a couple of years to one
see you have an idea OK this platform is

will be geared for
a trade a certain disease and

you sense a slowing down of particles and
then every day of the period and

characterize them and
feedback and go and go and

once you make sure they're not the poly
goes to work since the side and

the optimal point you find then you
can do some embitter evaluation and

it will take some more years
because you want to has that and

get all of those small Arab or
with different types or types and

some point you can get some fail here and
then you will do the same thing again and

again this is who entire
iteration process and

then after that are you sure it has to
be the small animal large animal once

you make sure that this is
working well it's not enough and

this is the fix a C. of the targeting
then it's time to secure this for

a human body task and this entire
Now days it takes a fifteen years.

So one of my posts out Barger he said he
created a platform nine hundred ninety

eight and still is not the approved
even though they believe that's really

not a particle and not a matter since.

So in my lab in my research
interest is how we can

make it IS challenges opportunities.

So the kid we want to make highly
reproducible in our part is

one thing we can think about
because the critical challenge is

to be very sure once you make a lot
of public today and tomorrow.

Those about US has variations.

So the kids how we can make this.

Continuous process like you get a car or

a certain product from
the factory large scale.

And we need to get the most people for
uptick in than those agents

in the non-apologetic trying to
follow the current stream particle

product and finally we also
want to make this kill this

so we can in in include dispensed opt
in system of cats radiation process and

scale love with this one process making
multi-functional materials in large scale.

And another thing we can do in
a lab is how about we can make this

evaluation process much more similar
to what's happening in vivo.

So we can we can make to the platform and
the system physiological lovable and

so for example mechanical
actively environment and so

we can control the properties of
mechanical stiffness in years to serve

the metrics pretty well homogeneously and
then we can also make a special temple we

control chemical factors not only Sure
stress but also what's happening in

the beta side of your cells and
between yourselves and electrical signals.

So this is the wrong term goal or
what we are looking for

looking to and we believe this two
steps really helping contribute

to reduce the duction the entire clinical
development of this and on the medicines.

It's sold on some go to today.

What I'm going to tell you is two aspects
in that pain in terms of manufacturing.

So we can.

I'm not going to show
you real manufacturing

none of my distance today but
I'm show you a way

the approaches using microchip a little
larger scheme micro for the chance.

And how we can get a better potential for
the non-manufacturing and

the other part I was show you how we can
test those normal particles in a Magic.

Mike when bargains simply mimicking
the end of supreme ability how we can

actually study the translocation of
nano medicine in vitro so we can.

Save our times and save our money.

Before going for the mysteries.

So let me go forward this to topic for
the first.

So this is motif company and
look to phones and other matters.

There are different shapes and geometries.

We can you can also work on
engineering the surface chemistry and

there are different type of platform and
you can combine some of this may get one

from unique and
take advantages from both platforms and

you can incorporate this
in certain not write ins.

To get a certain cells and
bodies to trade a certain.

For the same vision and also.

And as a protein a peptide so you can also

induce a search engine expression change
in your cells and when you bring a certain

acid in like you are in
a anti-microbial or a certain D.N.A..

What I have been doing is I'm I'm
choosing a certain platform and

trying to make this process
better with my microchip So

this is the hives I have been
developing with my microchip

with the same microchip the changing
the optimal composition.

So like I said the force of
the field by the ears and

pulling out apology with a surgeon
anti-bias following prop.

And you can you can get
the both the bandages

with this little point on the particles.

And I'm going to show you the high density

protein of the Constitution
in my microchip today.

So let me focus on this to perform
today and how I make the system.

Better to synthesize this now part.

Some of my not really familiar with
look at the particles look for

another particle is like
a hybrid now partly it's

the advantages from two different
platforms point in our politics and

life assumes and pulling out of
politics like you mentioned is good for

cooperating hydrophobic drugs which
is limited in life and reaches for

the poor worse all drugs and usually

most of the side of Terri cancer drugs is
high the phobic so this is important and

leaping the layer between the core and
shell which might be unstable but

when you think about this to hide a full
week and this is either a full week.

So this will make the system will

promote the drug with tension and
finally in has capsaicin efficiency

and with the bandages you can
get the serum stabile really so

hydrophilic shells it with like I said
you can make this stable a longer.

Stream circulation.

So this is two different times over.

Particularly since the start of my study
let me show you before going forward or

I'm going to show how hard to get
highly reproducible down Pollack.

From the bench top
conventional precipitation.

So now the precipitation is one method you
can make the leap and on the particles.

So you can get the whole member and
the full with drugs in all.

Face and
dropped is to the face of the lip it.

Why we are using drugs why addition
because once you add a certain

amount more than a certain amount
the polar is really easy to work again.

So you cannot stop the we gaze in that you
cannot control the side you cannot get

homogeneous Now apart.

So we should use this drug wide
which means depending on the people

practitioners how small we should use no
one knows they have their own protocols.

So that we'll create other vacation.

So like I said this is for
reproducibility it's to expiration

not just the process and
because this is look simple but

there's another thing between the way for
certain time and

then in a live it over and strain to
make the system stable solution stable

between organs just is and
how to control the mixing process because

drop is a strong we can control that's off
side with this man you're preparing and

it's hard to incorporate
multiple complements and

if we want to issue the ink and
insert as more steps.

So usually takes likes a day or
a couple of days and

most the first reading port is
interplay of the properties.

I have all other qualities and MIT this
is a sign of a particularly people now

are probably still there doing this
because this is one of the best

approach you can make a high quality
people know Pollock holes but

the question is even I am doing
the same problem just today and

one of my colleagues doing
the same composition and

there is still very ancient but the
problem is even I change the composition

from one end to the prophetess
is not independence.

So if you change a certain.

Because.

Particles.

If you change the Lippitt concentration
and pull them a concentration

a certain type of the target and
proteins all this content ration and

composition will make this
one our particles and

those compositions into play
each other one another.

So once you change one you should
think about how this guy effect

the other composition change.

So that's a frustrating process
too as it's very hard to find out

which are fairly different
type of properties

many people use Mike to further if to
since the side pulling on particles.

So I'm not sure how many people
are used to the micro for this but

once you control the range of the number
pretty low range then you can

get this simple straight for
streamlined without mixing but

the fusion still works you keep the rain
at the number of less than one rest and.

Sometimes differently
depending on the German.

Cases in microfiche al.

So you want to control the flow
rate you can control the with and

the feel the pad pull the particles can
be says a size across this interface

so you can make it a little faster and
smaller and control the with and

depending on the narrow part of
the properties to your God You can get and

optimize is just the problem if you can.

That is still some longer
than a couple of hours.

Why is that because like I said
pull him or is is it's a brigade.

Once they are in a steady flow condition.

That's why we stirred the solution when
we make the people in our power tools.

So why is there because even though you
make a flow usually pressure the of.

Microfiche L. has closed the profile.

So you're in year the war or

real brigades over time and
finally clothes challenge.

And some people working
on the same process.

This is a diffusion basis is this what
about how we use our come back so

they use come back.

Ssion a certain type of mixture inside
there are a lot of different type of

conversation I mean Michael Food Channel.

So they can develop faster and

also a little poem around Apostles
which cannot be fixed by diffusion but

still the same problem the poll
him against because they the now.

So what they develop in the same lab is
OK we can make this really focusing and

so we can keep the podium or
from the center stream on the center.

So we don't have to worry about to
put a motivation and it worked out so

they use this device to synthesize
probably cols But still to me.

They're using the fusion.

So once they mix using
a certain chaotic mixture.

We cannot guarantee the poem or
a gate or not.

So this is still limited
to go through which is

our the reason I'm taking
the number here is our means

we need a certain a few grand for
one mouse studies for did is we

need to make this like several days
several weeks just for a couple masters.

This is the month we are making use of
microfiche I know once you use the fusion.

But there are many people
working on this fully

the mechanics that flow parents know
how about we can change the geometry.

And using a certain convective mixing or
sometimes in a show of force

in microfluidics So this is the I
call the secondary flow formation.

So once you make a search
in the group because I was.

Bottom of your microfiche.

And you can get the circulation flow
on this from the certain faction and

they will help you separate
the micro particles.

And sometimes they can sort.

The particles depending on the flood
because we call this the in for.

OK mate is secondary flow to simply
make the channel curved like this and

they can optimize the length
of the channels so

they can get is like
a Pollack who separated.

Which is impossible for a nano particles
which is one thousands from over.

And this varies with changing
the just size actions and

make the stream focused on the center.

Which means that we can use this
was we can mix the nano particles

to the political solutions because we can

to avoid the problem of creation and there
are some more high suppressed or they say.

Using some of the homes and trying to
analyze the particles in the center.

So one thing I found is many people
using simple three in that channel and

you can see with us
a certain range number.

Which is a little small fifteen then you
can see this in your interface between

these two was the use
of blue dots just three

in the red on the sensor what happens if
your stream is pretty straight forward.

Until you clear the height of one hundred
while before you increase the high

three hundred and
trying to maintaining trying to maintain

the arena's No fifteen we can find
even that small they know the number

the microphone Pad and
the whole forwarder.

So we can see this kind of pattern.

One of the reason is because
is this how to stream.

Can create a certain separation
wants to increase the.

Height of the channel and those flow
pattern is three dimensionally sensitive.

So not many people using this power

because it's hard to insert a new
inserted to be in the micro scale.

But what if we change this.

If we change it is simply just directional
The you can change it totally up

in the downstream which is focused
which is very useful in many

occasions I've only seen societal
walls to match very well happen is

you will get the separation and
the intersection

and that separation can create
a search of the circulation and

a type of microbe Otis's So
this is the what's going on in

the section and there's a micro
vertices in the corner in the here and

there was a change in
the edge of the trees.

So I thought what if we use this mark
of vertex to be steered to because

solutions and still maintaining
the focus impaired on the downstream.

So this is a simulation stranger
simulation so I can control.

If I keep following this number ten.

You can see it is the Fusion and
the in year two for

particle living here parents but
one seeing Chris ring there's

a number from ten to two hundred
you can see this different.

Pattern transitions a certain point
you can get mixing pretty well mixed

situation between these two streams and
you can still get the focus impediment.

So I use this device.

Which has a four hundred
my community hall.

And all to monetize the micro boards
experiment to synthesize the people in our

part and the Chivas through
simple three unless parallel.

And you can see is up to
one hundred one thousand.

Runs faster in terms of process
rate is a cramp or hours so

you can make another particle
in a continuous process.

For an hour for one mouse thirty
which is totally different situation.

Environment when you
have him be able to this.

And yes we can we can avoid the problem of

creation with the by one or more of
the bears we can take from these devices

you do what we need to do once do you
want to change the size of particles.

You should change
the composition Lippitt and

the poem or some depending on
the your platform but this devise.

You can change the side dynamically
once you change the way numbers.

If you keep the repeated
poem a competition at hand.

Just change the rain is the number
you can change the size of who it

is big deal because size dependent.

Since this is takes days and
weeks and months.

If you want to get optimize competition.

We have still some
property quality issues.

Depending on the Brainard's number but
you can still figure out why

this is not near because of circulation
patterns in Offaly developed so

once a week hip a certain range of rain
has a number we can control the size of

not a particle dynamically without change
the composition of little importance.

We also tasked is how much
how many multiple drugs and

imaging energy that we can also.

Combine in this same microchip.

So dogs stary for
you can kill the cancer cells with this.

And as our rafts and try and Janick.

You can carry your understand cells
come down so close crystals is a good.

You know for extra microscope and
city detection and you can.

The Using pegylated and Tyree by founding
properties and sysadmin for near infrared.

Fluorescent imaging.

So we have your model will be
able to pull up to compounds.

So we can see or
read the rate is hydrophobic.

Characteristic in the core.

And this little bit and this in across
the shelves of the people in our part.

We are using a third sense of
the ship because solutions and

using the optimizer
microfluidic patterns so

you can get is the people not
a particle you can see all the calls

captured inside with very
dispersed the opposing one.

And we can target a certain size
eighty five or seventy or sixty five.

Based on this device.

So we pass the performance of
the particle we developed so you can see

the drug will this which is depending on
where to look at it in your now particles.

If the drug is in the shell you
can get a faster through this.

If the drug is a coup or
you can get will slowly drove this.

So we found the point that
is true is in the same

which is on the day twenty four hours and
we tasked with with that show you.

For that time period.

We have one of the best kind of cells and
the cancer cells and

tested to prefer of these drugs and
you can see the Depending on which drugs.

We use in control and
just down the particles and those ones and

ones are multiple draws you can see
that to perform a combined effect and

you can also see the C.T. mages by
see theme it is all non crystals and

images for size seven.

We also value that is the approach we
inject if we use of a injection of.

So you can see
the accumulation of apology.

In the mouse.

Let me move on to another of
another popular toy we created

before going forward we all know
what the high density lipoprotein is

it's good cholesterol
because they transport.

This we saw cola lever's
cholesterol transport.

So it's there will help you to remove
a certain Lipitor accumulation in your

blood vessel or on the cat heart area so
it can help you.

Make your healthier in terms
of your vascular disease.

So this isn't that sort of you
have your eighty and your body and

this which means it's very stable by a
comparable biodegradable in the genus and

also long stories alive have a search and
quest for and so

I lifted a ship in the rules to distill

it can be the constitutive but
just by Batiste process.

Let me show you how Usually
we make the heights.

So we have lifted an affable and

crystals because we want to
track where the allocated.

So we are using an imaging agent and
depending on the cat's

rest of imaging agents we can
use a certain already servants.

So you can get the mice still and
there you were you can get

this this time because today to
do a case you can just add this

leap into water induced a step
assembly of my SIL's and you can and.

And April like a protein
a want to get it and

in this case you can do or
more times it's a bit Crystal.

So you can get another my cells and and
of course for encapsulated in my SO and

then you can the protein again.

It is cooperative they consider.

It has like twenty four hours of video
Boreas you should stick to it all

the process.

So we did the same thing we reconstitute
H.E.D.O. the same microchip the changing

the optimal competition the flow rate and
you can see

different materials we can make.

So we can make just simple disk.

This is called the edge of the L.
we call the microwave studio and

you can also incorporate
the four big molecules and

step in which is why the four drugs.

We can incorporate call their own and
contemned out for their emissions.

So we have to ask how fast we can
make compared to the conventional

benchtop process.

We almost up to eighty times faster and

you can see them of
course is very similar.

All right Sibila present the cost is
actually the early constitutive by

the bench the process and in terms
of you know the steel we are a child

who are you know we have a problem to make
you feel better but Michel for the cello.

This is the same because they see
their process and we tested this age

in macrophages so you can see
the cluster afterwards microfiche is.

With this our microphone and
the human plasma.

We call in nature and

the conservation of issue of
the similar cholesterol afterwards.

So we think this is.

Michael.
For the benefit of years prior to two.

And we can also like I said we can
incorporate hydrophobic mole tools.

So we can detect this H.E.D.O. when
the cell takes in vitro and we can also.

Incorporeal hydrophobic through standing.

So you can task pro in private to factors.

How was to you to see from
Asia of the Mecca fish which

is pretty activated and
Everyone's to we add the stereo very well

you can see the reduction of
the seclusion from the metaphysics and

you can also see that all incorporated
in hindsight incorporated the else.

So we can use this in C.T.
erector microscope.

And M.R.I. to summarize in this part we

successfully produce the now
part of a large scale with

the potential manifest
manufacturability of this material.

So we use a single still assembly which
is important in single layer a single to

platform the still we have
potential to tell lie generation.

So our personalized gram per hour can
be killed on power was a business

that was to make this largest
scale integration system.

And with a certain precision
control of this multi poor

single chips and we can also incorporate
multiple throws imaging agents.

So we can create much a company
out of the three platforms.

So let me move on then once you
make the nano particles and

how we can test this better.

Compared to the M.B. traditions.

This is in general

might by a man Microsystems like trying
to maybe a certain structure and

functional reading systems
are what we need is

we need a certain three the architecture
because we need to think about

what's going on over the cells what's
going on beneath the cells and we my want.

Coacher different types of cells and
up and down near by.

Three The synthetic scaffold
is very important and

we need to control
the mechanical cues like for

example shoes dress and
the stiffness of your mattress.

And then we should control
the chemical factors and

not only the nutrient
a certain type was to lie.

And we should also keep the electrical
potential which is usually

open look before entire system issues
to control the article as well.

And all of this control
sure the be spatially and

tamper with a delay of
the many people trying

to make a certain we call so-called organs
on the chips or even the human or chair.

This is two thousand and ten and
science they use a simple

layer market share with the host
membrane and they use a vacuum pump so

they can stretch and contract a membrane

which will give the end still cells
stretches and constructed over time

which can mimic a certain function of
what's happening inside your loan.

And this is a letter to stimulation.

So you can create a certain feel.

Which is muscular thin film so
you can connect to cigarettes.

There you can stimulate that and
you cane use the contractility and

you can steer the work environment well
kind of situation you can get that

kind of contractility A muscles and you
can also develop certain basic Larcher.

And wrapped wire colors in one and

you cane views the street the sprouting
which is very important in terms.

Earth.

So they can study and try and Janick.

Phenomena with this microchip.

And now these people are also working on
the integration of all these are going.

Whether they're focusing on one organs and
their function and structure.

They're interested in integration.

How many oxygen.

How how high concentration all season and
pressure and

carbon aside a needed how we
can control this pretty well in

a search and this entire systems.

What I'm going to show you today is
one simple the idea of microchip moral

they can mimic a permeable and
the ferry which is very simple

very important if you want
to control the liability.

So let me show you why this
motivation was me what the beige and

come from this is a terrible process.

If you have process.

There's a nail vassals can penetrate
being there a certain type of the bait.

When you treat other courses.

Can you just leave or
your nano particles in this large

other is this blood vessel or

this now Paula cools can be the reverse
to the nail vessel in the other side.

So what we think is out
there might be both ways for

many people believe that it will not
happen through the naval vessel.

So we tested with this
hypothesis that the particular

cumulation is full force of
the twenty that by highly

permeable now baffles penetrate
into the plug from the network.

Michael Bessel the supplier
the world's largest.

So the aim of this is develop a simple
in-vitro measurement of underserved.

Well maybe once the you
stimulate in the Syrian

pour in forever from A to tractors and
control the show stress patterns so

we simply develop a poorer and

lower layer and
inserted a Michael poor membrane.

And an incorporated lecture.

That idea we got from even one if
a commercially available device and

then we got is devised with
the Michael Jackson embedded so

you can culture cells and
trying to get the video depending on the.

Year in the frame over time.

So if I.T.C. alchemy is common
combination of mess of the brain you.

Check the ability of your mono layer.

So we got this correlation simply
we changed the problem of media and

we control the speed of said growth and

you can see that goes up and
the F.T. album in.

Was We have higher population of industry.

So we try to control the in
the supreme ability.

You can.

Once you cut yourself with this
in device you can get this part

after two days and we can think

about this model layer over in the stadium
cupboard all the membrane of years.

Microchip and then we can do we can

turn a fall for and we can also
see how the different was to be

stimulated this pro-inflammatory factors
and your body and the ceremony layer.

Has higher probability so you can get more
translocation means in both ways we can.

Make sure the pony ability how things go.

And once you take images.

This is normal and the cells when they.

Were established a model a year and
once they stimulate

certain information factors
then you can get disrupted.

Just serve.

Interest or at junctions.

So we says Assad using our device and
nano particles which go in and

five five foot fall because we want to
make sure that the pope has a correlation.

With the.

With in my kitchen.

So you can see it is limited so
we have the people in our party.

It's a durable deli and we succeeded in

making the scale production
up to some readers.

So we can use this amount of
Not A particular in various cities.

OK.

So what we did was we have mono
layer of then the stadium.

Then we change different tiles your stress
disorder different level of sure stress

from Point want to tan and

then we added to their fault and

for ninety minutes with this
difference your stresses and

they really move to they fall for
a little bit another ninety minutes and

see how they change and
then we suspend our politics.

And you can see this after three hours.

How to you are very good and

how what another part of
the train is the occasional.

One.
So we monitor it.

T R Every ten minutes and we.

Sam pour the now particle from
the low Traverse so we can compare.

The nanoparticle translocation and
T R And really pitted this experiment.

We got some correlations
that intuitively correct.

We increase the ones we have

similar to your Which means higher
premiums really you can get more.

And this is all the data point we go.

You can see the jump when we change when
we got the higher premier billion of these

small a year and you can get
the translocation higher not a particle

translocation and when you look at
this this is a four to five person and

this is less than one percent.

So this is a very high priest highly
precise control of the system.

Leather then what we usually do we fix
the cellar up to the experiment and

compare how we could.

But how we can really say
dynamically best selves and

their situation hold forward and
measurements.

So in that perspective this is important.

This to this important.

And how we want to validate what we
guard from Microchip in large animals.

So this is the No more rabbit and this.

Diet which can induce

the article closes you can see
the premier ability using this M.R.I..

And was excited and
fired this error that you can see

the difference depending on
the site you can see the huge

difference was we have the playing
ability in this chorus and

this is the more closer the images
into yours that this is no

more underseal self and
keep the junction closed pretty.

Well I was this is in this
situation permeable you can see

this permeable between this and
the this is two microns bar.

And we use the same batch
of down the same day and

doing the same Still the in-vitro
in Beaver and we got to control

part two cases using
the five point five five and

using size Fairbairn we can get
their intensity separately.

So we use all the mean for
permeability test and

we use another Politics various occasions.

We did another part of the test
cation using our people in our parts.

So you can see the correlation
between these two when the cells

permeable the mirror we
can get more particles and

we got the same thing same
data in reaching the same.

And we got the same correlation with.

And this is a curve closer
images is the normal Basle and

there's all fluorescents so
you can see the vessel war and

if we have you can see the nano
particles accumulate in the plant and

you can also see the micro features in
the silly and the serious cells and

this make a fish uptake of the particles
more importantly we see that is now

on the macrophages nearby in
their vessels which is far

from the Raj their phone other is so
we can think about this now because.

So it's OK to through
the narrow vessel permeable.

To summarize we've developed a simple
microchip with live monitoring and

to see your.

Homie ability and
we enable finally measurements.

When the supreme ability and we believe
this microchip is a unique tool for

the nonpartizan occasion.

So we can screen.

Nonna medicine for other teachers too so

we can control the pressure
the French are.

So we can control the pressure
differential across the barrier

because usually the cancer that is a high
interest feature pressure difference.

And also.

We can we found in our politics
as a case of for plot our giving

a course at the site increase the
probability and also the lunar cycle and

obviously the real war and
disintegration imaging.

Show the similarity between
the markets and the rabbit trail.

So this is one project our lab
is working in Georgia turned

the previous test of this which
I have done in MIT and is post.

And now we are working on developing
cheap little micro for the.

Vessel one of the reason is
if you have a right angle and

rectangular cross-section like a for
Channel.

User and the seals of though I
like the color and that means they

will not really making establishing
well established in the layer.

So we are heading for
this and still me trying to.

Measuring the CIO are not only measuring
the premier beauty but also trying to

train their viability in the incubator.

And also we are going to
control issue of stress.

So we can study how the novel
pilots were created

as well as interacting with this and
this is.

So we can monitor.

The T.V. are trying them equally.

We can change the chemical factors.

So we can increase the probability
of certain in from radiation.

We are going to see particles
developing new platforms microchip and

try to optimize the flow patterns for
different types of public to types.

Thank you my mentors and family members
and collaborate in Georgia Tech and

the father who searches for
the resources and the funding from MIT.

Thank you for coming and
take your questions if you have thank you.

So just

right.
There are several

ways you can characterize the rain
there's a number locally.

But in this is what we did is we

just based on the entire volume
flow rate through the downstream.

That's the way we calculate to enter
something where we are trying to find

some localized effect
that will need rain or

to number all the other number
how we can actually get some

correlation between the certain flow
types and the nano particles holding.

Thank you.