From chest curse toward a chair assistant
professor at the school biology of

Georgia Tech.

The goal of my lab our understanding
mechanism of D.N.A. repair and

developing more approaches so for
gene targeting using D.N.A. and R.N.A.

molecules the inner repair
is a complex of D.N.A.

transaction necessary to maintain
the genome integrity of cells the D.N.A.

of cells is constantly threaten by
exertions and indigenous factors.

It was factors such as radiation or.

Chemicals and doodles factors such
as a reactive or social species or

the facts of this kind of core during
the eye metabolism so all these factors

can cause lesion into the D.N.A.
of cells and this lesion should be repair.

Because if a repair does not occur or
is not precise.

Then this can cause mutations which
can lead to genetic the fact cancer or

premature aging gene targeting
it's a complex of D.N.A.

transaction important for
modifying the genome.

It's we're in the laboratory and
both gene targeting and

in Europe they are most
often are involved and are.

Based on the same D.N.A. transactions for

understanding and
course horizon better mechanism of D.N.A.

repair can help to develop
a more powerful and

more efficient procedure to
modify the genome of cells.

And the implication of gene
targeting can be enormous.

If these are directed to correct
genetic differences that occur

in the genetic disorders such as
disorders who are just acquired from.

Fission is.

Affecting G.M.
and causing disease in humans.

So correcting a defect directly and
in D.N.A..

These assure the stability of and
normal transmission of the.

Correctly in a way to
the next generations and

also sure natural expression of that G.
and.

We because it was Jimmy ears corrected
in its And the original was luckless and

moreover this can be the remedy both for
recessive and dominant.

Defects some However gene
targeting before we can bring in

a strategy for cure or

prevention of genetic diseases
must overcome several obstacles.

So it shares the if issues of do
we worry of the correct him or

Q To the cells and
the efficiency off the correction process

itself as well as the safety
of the procedure and

start is in more the systems
are very important to achieve these.

Goals and
the more the system the way our work you.

In our laboratory are the unicellular
eukaryotes are used as

a Karl Marx's receiver and human cell so.

In culture.

One of the Morse dangerous
lesion in D.N.A. our.

Breaks ranks above us and breaks into
D.N.A. because even if a single double

some break it cannot be repair
this can lead to mutation or

trigger chromosome rearrangements that
can cause cancer or premature aging.

So the cells must look for a system.

That can repair this break
as precisely as possible and

the safest way to repair a dumbass and
breaker is by using.

A template or with them.

Ology to the broken D.N.A. answer.

So.

Because just like getting
the broken answer.

Through normal course and joining this
can often be mutagenic because if

you basis can be lost or if your business
can be incorporated during the process of

repair instead of want to sell
confine in a model that's Mark

you to repair it is break then
it is broken the broken and

can invade and
a model goes duplexer of D.N.A. and

retrieve the information
necessary to refer to break and

up to now it was known that
to break into the IN A can be

repaired utilizing only D.N.A. template
and what I have shown in my post the cross

study is there to not only D.N.A.
but also are and they can be utilized.

To repair a breaker in chromosomal D.N.A.

and this was shown in the smaller
system used to succumb ISIS or

of easier with the use of
only going to critize so

short of stretches of single strand D.N.A.
complimentary.

To the broken region off a marker inserted
in the genome of Easter else and.

These holy going through time were
designed to repair the breaker

in this market and
to restore the function of this market and

here we see different all going to critize
and Louis D.N.A. and red is aren't any and

each of this all going to try to
contain a different slant of irony and

it was thought that maybe just
having your base or so on and

they would completely prevent
the repair of the break.

Instead of the present.

So far and they didn't abolish repair for
personal core and the official seal

prepare is the creators increasing
the length of the hour in a truck but

nevertheless even an hour and
they only mark you can report breaking and

deficiency is lower compared to D.N.A.
But this is mostly due to the addition

of the R.N.A. only going to a tide
in the cell in fact protecting

the end of our in a all we go
with the tail of D.N.A. that.

Is aspecific that can north.

But dissipate in the repair of
the break increasing efficiency.

Almost three hundred four.

So this shows that are not only this are
in a Mark you can repair the breaker but

can also transfer information
to through the chromosome

because this all goes contain
changes that are not to your D.N.A.

that are not present originally
when the chromosome.

And we can then detect these.

Changes.

By sequencing the D.N.A..

So the men finding are that are and
they can repair that was from break and

moreover can transfer directly
genetic information to a gentleman.

And this not only happens
in human cell but

what we have shown now in the lab is that
this can also occur in a human cell so

where we are in a containing I'll
go look at diets we can repair

doubles from breaker in use in
the group for us important marker

gene in the chromosome of human cells and
we can detect to repair by.

Looking and identifying groups for us and
to sell so this bright cells here.

Moreover also irony can transfer
information to genomic D.N.A. in but

cure yourself as we show in its cricket
call it an R.N.A. containing only go.

Can.

Correct the defect in the legacy gene.

With are only five percent efficiency less
than a corresponding D.N.A. molecule.

The four are in they can have a direct and
active role in dealing with if you

question and remodeling from bacteria
to my mother in human cells.

And what we are focusing now in the lab
is to understand this mechanism.

How does our in a repair.

D.N.A. how this transfer
of information a core.

What are the factors that are control the.

Has to be with you.

Of the hybrid between D.N.A. and R.N.A.
and what police arrays are involved in.

Retrieving the information from the D.N.A.
from the are in a truck.

So the prosperity of our That stable
transmission of information from our

innate to D.N.A. make contributions to put
genome integrity and very efficient in

this capacity of R.N.A. to record itself
in the D.N.A. genome could be the basis of

a completely unknown process of
our in a driven D.N.A. I will.

And in addition to development of
strategies the export of such unique

are in a function may also have
important implications for

the development of novel
approaches in June targeting and

gene therapy such as for
example a splash an off short or

are in a molecule like a short transcript
that can be designed to correct

the facts in the chromosome and
the advantage would be that this.

Are in they can be amplified within
the nucleus of a cell because

they can be transcribed in high amount or
the transcription can be regulated and so

then these could modify
the genome can have the chance to

modify the genome of cells and
also focus in a world lead.

The identification of.

Fructose so that can facilitate
delivery of targeting molecules

to the nucleus of cells and promote
the exchange of genetic information.

And the so our I will focus is the
development of not only fission systems

but also system that her for
correction of genomic the fact that her

would not affect the genomics
the beauty of the treated cells.

The result of their actions are in
summary to determine the mechanism

how our in a business can transfer
information directly to genomic D.N.A.

and also to to task.

If you can see that that are in they
can also transfer information or

repair a genetic defect
directly into cells so

an irony that is generated in the cell.

We hope prove that with the use of for

exertion those are in the way that are and
they can serve as a template or

for D.N.A. repair Well now we want to
see with endogenous are in a safe a cell

can use its own are in there in some
circumstances to repair D.N.A. damage.

And moreover to develop novel and
efficiency and safe approaches for

Jim targeted with potential procreation
engine therapy with the D.N.A. and R.N.A..

And this is a my research group.

Judge attacker with very talented and
motivated Research us with a dog and

the three graduate student to
serve our undergraduate student.

Thank you very much.

Thanks.