So the top of my presentation
is a mission dependent change.

So why is it important to study
is an essential trace element

that's involved important cellular
processes such as growth regulation and

reproduction biological structure and

regulation of molecules in the cell.

If we look at the content

there approximately ten to
the power of eight per cell and

you carry out according to them or
you carry out Excel seems insignificant

However when you look at the volume of
five hundred from two readers we find that

the concentration is quite significant
point which is relatively high compared to

the extra concentration of zinc
which is a micro range zinc and

port cities have shown as an important
export are tightly regulated.

However most zinc in the cell is down
to protein how are these proteins

fast exchange with their environments
which indicates the presence of kinetic

link or using terms training methods or

so we're seeing a section
of the brain of a mouse and

the darker regions indicate higher
concentrations of zinc look closer at

a particular region their
black regions indicate

and we can't worry about testing with just
a chemical technique which is right for

what we'd like to use it for our lifestyle
imaging and campaigns for live cells

which is our ultimate goal we're
going to move on to using.

Possibly for sent probes are popular for

certain probes that bind to zinc and
possibly fuse into the cell and

the enhancement that indicates that
it's complex and balances Inc This

is a person images that works and
also the campus region.

OK are relatively the same
strain patterns and personally.

Are dependent not only on
the concentration of zinc but

also on the concentration as well as in
combination conditions conditions and

a host of other factors.

So how can we quantitatively determine
the concentration of zinc in the cell.

This probably has already been solved for
the calcium care which is

using appear to center binds to
calcium and exhibits a shocked and

excitation spectral molecule looking at

looking at two different wavelengths
the ratio of two different wavelengths of

the spectral information we
gather is completely independent

of all the other factors So this is only
dependent on the look in concentration.

There's only been on the zinc concentrate.

Karren very calcium current concentration
is bound to the filler to center

which only it's all the other factors
that we had to deal with before.

So here we can dynamically volume
the changes and calcium in a cell.

Unfortunately this particular approach
is excitation metric only and

not emission ration the tracks
are exhibits a shift and

excitation spectra of the pro but
not a significant shift in the emission

spectrum of the protocol in complex and
calcium.

So how do we solve this issue
design evolution racial

naturalism sensors depends on several
factors most important as a measuring.

Tricks and some property of the pro and
this is due to the high cost of

excitation metric sensing other
factors include optimization of

cross section tunable affinity cell
coming ability in on time and control.

Find some cellular distribution
such as in various organelles

so excited central to our Proton
transfer of studying this.

What happens here is that
this particular molecule.

Upon excitation the proton transfers over
to adjacent space which in this case

but after observing a photon of
light it undergoes excited state and

from local proton transfer proton

emitted as a large structure

gives off a large structure to the mission
and Thomas's back to the ground state.

However when this particular molecule is
bound to the metal ions such as ink in our

case this process is inhibited strongly

emission of looking at the differences and
edges of the missions between

these two different processes we can tell
when the probe is bound to matter and

when it is free so
our particular case we study of the bands

of it is all you just saw looking at this
particular graph of the emission time for

the decrease and listen mission time
since the mission intensity and

simultaneous found emission
intensity of the probe

so previously with substantial
effect on our one which in fact

properties of the probe and also to
the binding of the substitution of.

So now we're looking at substitution.

Dr which principle of tuning
ability of the probe.

So here we're looking at the molecule

the picture of the molecule
you can see that the process

for some of the molecule in
the state lies on the bench and

that is I mean the final range and
not on the SO here.

So the first region is
just this region here but

substitution on the right kind of
effect for the inductive effect.

So we would like to see
a substitution effect on one of my and

its influence on Proton transfer kinetics
changes in absorption emission and

influence on quantum euro which is old and

handsome and
not related to activation pathways and

for quenching So looking at the synthesize

series of twelve derivatives from
commercially available religions and.

There are groups of this
abstentions it is religious and

can be characterized by their
habits predators and increasing

value correlating to withdrawing
nature substantially.

So looking at our data we see
the absorption spectra of the compound and

about three hundred and
ten nanometers an overlay of

the spectrum of the other situation is
a significant shift in absorption so

it's not influencing there was an option
spectra of our compounds significantly.

When we see the highly structured
remission of the same unsubstantiated.

And other derivative shows that

there is no significant shift
in the mission as well.

So it's not influencing our for

the physics as we started night
through an inductive effect.

So looking at the call.

Actually there is a higher
variation between the neutral and

basic condition of all the derivatives.

There's no obvious trend
that's occurring but a product

of the quantum notion of the significant
correlation for the middle range and

outliers are the strongly with strong and
strongly donating substitutions.

So this indicates that a possible problem
to select on transfer pathway may be

occurring and this might occur
from the first time it is all

I watch on may transfer over to
the cell phone of my derivative upon

a substitution of a strongly
withdrawing when there's a strongly

for doing research on transfer me
occur in the opposite direction.

So in this case the molecules
excited to as one state

undergoes a foetal induced Ultron
transfer which results later in

totally combination which quench is
the mission of the molecule this process

is characterized by
a change in the transfer.

So we looked at our formalism which
says that the change will free energy

a result of the donor oxidation potential
except the action potential and

excited and
in our case we didn't affect or

change the donor oxidation potential which
was the thing that is our region here

the only influence was the substitution
on the accepted mission so

we focused on this factor and we did so
by going through clinical studies and

using cycle we see a cycle
time of the compound

at about negative point for
both looking at

the subsidy strongly withdrawing some
situation we see the same negative points.

But an additional third and about
negative two point one which in the cell

phone of mine is being
reduced a correlation of the.

Action potentials are compound parameters
and provides a strong correlation

with the same outliers as before the
strong withdrawing subsections looking at

the quantum chemical calculations of
truth to do with us on this side of

the compound excited excited state for

the answer with possible
electron transfer state

occurring at this formation before
the scientists have stated to look through

transfer states are present
as one was in the case and

upon excitation the sign of
compound undergoes activation for

them to select on transfer and quenching
emissions which supports our data.

So looking back at the quantum
you explain quenching

strong with strong social situations and
personal

and we can see that most of the compounds.

There's also a question of emotion which
is expected because of the decrease in

oxidation potential which increases
the delta of the However this can be

recovered upon titrating with zinc and
hence is the mission of the probe.

So in this spirit we can see
that some particular molecule

emission only molecule simultaneously
as the zinc down emission which is

from the increasing this particular probe
is quenched under basic conditions.

So in this case the molecule
the complex is independent of changes.

So looking back you want to diagram
that upon excitation printed.

Molecule by molecule undergoes and
that's largely served a mission.

However when the president
is trying transfer

combination which crunches
the presence binding to zinc

is that quenching is a force and
provides a strong signal.

So in some way the electron
withdrawing introduced the quenching

of the department of zinc and

allows emission combination of
opportunity to design insensitive probes.

So long technology started
actually surgery and

Professor Frank as well as and I

mean yes

they were in methanol and

water and water and
everything very solid and

still in the silence of this
case I was studying just

such an effect on that particular ring and
wanted to eliminate any other variables

you know actually Professor
prior to the calculations.